VEGF binding to NRP1 is essential for VEGF stimulation of endothelial cell migration, complex formation between NRP1 and VEGFR2, and signaling via FAK Tyr407 phosphorylation
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VEGF binding to NRP1 is essential for VEGF stimulation of endothelial cell migration, complex formation between NRP1 and VEGFR2, and signaling via FAK Tyr407 phosphorylation
In endothelial cells, neuropilin-1 (NRP1) binds vascular endothelial growth factor (VEGF)-A and is thought to act as a coreceptor for kinase insert domain-containing receptor (KDR) by associating with KDR and enhancing VEGF signaling. Here we report mutations in the NRP1 b1 domain (Y297A and D320A), which result in complete loss of VEGF binding. Overexpression of Y297A and D320A NRP1 in human u...
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The vascular endothelial growth factor (VEGFA, VEGF) regulates neurovascular patterning. Alternative splicing of the Vegfa gene gives rise to three major isoforms termed VEGF121, VEGF165 and VEGF189. VEGF165 binds the transmembrane protein neuropilin 1 (NRP1) and promotes the migration, survival and axon guidance of subsets of neurons, whereas VEGF121 cannot activate NRP1-dependent neuronal res...
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Neuropilin 1 (NRP1) is a receptor for class 3 semaphorins and vascular endothelial growth factor (VEGF) A and is essential for cardiovascular development. Biochemical evidence supports a model for NRP1 function in which VEGF binding induces complex formation between NRP1 and VEGFR2 to enhance endothelial VEGF signalling. However, the relevance of VEGF binding to NRP1 for angiogenesis in vivo ha...
متن کاملPhosphorylation of FAK on Ser732 is induced by ROCK and is essential for Pyk2-mediated phosphorylation of FAK on Tyr407 in response to VEGF
Focal adhesion kinase (FAK) is phosphorylated on tyrosine and serine residues following cell activation. In the present work, we investigated the relationship between tyrosine and serine phosphorylation of FAK in promoting endothelial cell migration in response to VEGF. We found that VEGF induces the activation of the RhoA-dependant kinase ROCK downstream from VEGFR2. In turn, activated ROCK di...
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In vertebrates, organ development, homeostasis, and repair rely on properly perfused blood vessel networks. The first blood vessels in the embryo are assembled from single-cell precursors, which coalesce into a honeycomb-shaped vascular plexus in a process known as vasculogenesis. This primitive plexus then expands through sprouting and remodeling to supply all tissues and organs in a mechanism...
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ژورنال
عنوان ژورنال: Molecular Biology of the Cell
سال: 2011
ISSN: 1059-1524,1939-4586
DOI: 10.1091/mbc.e09-12-1061